Cell Based Assays
Abcam Lipid Peroxidation (4-HNE) Assay Kit
Lipid Peroxidation (4-HNE) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc DNA DAMAGE QUANTIFIC KIT (20 SAMPLES) is a colorimetric microplate assay and can determine the number of abasic sites in genomic DNA samples.
Oxidative damage to DNA is a result of its interaction with reactive oxygen species (ROS), in particular the hydroxy radical. Hydroxy radicals, which are produced from superoxide anion and hydrogen peroxide by the Fenton reaction, produce multiple modifications in DNA. Oxidative attacks by hydroxy radicals on the deoxyribose moiety leads to the release of free bases from DNA, generating strand breaks with various sugar modifications and simple abasic sites (AP sites). In fact, AP sites are one of the major types of damage generated by ROS. The Aldehyde Reactive Probe reacts specifically with an aldehyde group present on the open ring form of the AP sites. DNA Damage Quantification Kit contains all the necessary solutions for detecting between 1 to 40 AP sites per 1 x 105 base pairs.
Miltenyi Biotec, Inc. FASER Kit - PE
For increasing the fluorescence intensity of cells labeled with antibodies conjugated to PE.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Miltenyi Biotec, Inc. Propidium Iodide Solution
Detection of dead cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 1 Reagent, 100. (93-0563R1A)
AssayComplete Cell Plating 1 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0859E3CP0M)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 2-Plate. (93-1045Y3-00043)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Grainger G0365 Toolboard Accessory Kit 1 in
The Wall Control Toolboard Accessory Kit includes 17 accessories to organize tools on a toolboard. It contains 12 small steel bins, 2 large steel bins, 2 steel shelves, and 1 steel rail. The small bins are 3 inches wide x 4 inches deep x 5 inches tall. The large bins are 6 inches wide x 4 inches deep x 5 inches tall. The shelves are 18 inches wide x 5 inches deep. The rail is 18 inches wide. All accessories have a black powder coated finish and attach to the toolboard with included hardware for easy customization. This kit is designed to work with Wall Control's 30-inch x 48-inch toolboard.
Dojindo Molecular Technologies Inc Liperfluo(50UGx5) is used to monitor lipid peroxidation in ferroptosis research, emitting intense fluorescence by a lipid peroxide specific oxidation, and is applicable for microscopy and FCM analysis
Liperfluo is designed and exclusively developed by Dojindo for detection of lipid peroxides. Among fluorescent probes that detect Reactive Oxygen Species (ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, both photo-damage and auto-fluorescence can be minimized. The tetraethyleneglycol group linked to one end of the diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Though Liperfluo's oxidized form is almost nonfluorescent in an aqueous media, it emits fluorescence in lipophilic sites such as cell membranes. Therefore, it can easily be applied to lipid peroxide imaging by a fluorescence microscopy and a flow cytometric analysis for living cells. Liperfluo is widely used in ferroptosis research, a research field Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Hydroxyproline Kit, 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Hydroxyproline Assay Kit enables collagen analysis in small tissue samples with low collagen levels. Quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. NOTE: Kit does not contain tubes and protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many studies.
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Collagen Assay Kit, 2 x 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Collagen Assay Kit enables collagen analysis in small tissue samples with low collagen levels. The assay is based on quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The assay allows quantitative measurement of all types of collagen, species independent. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit Colorimetric Method 1/EA
QuickZyme Hydroxyproline Assay Kit 2 x 96 Assays (includes 2x 96-well plates) The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues in a fast and simple 96-well plate format This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed This assay is the ONLY hydroxyproline-based assay that does not need this laborious step resulting in a fast sensitive and easy assay NOTE This kit does not contain tubes or a protocol for collagen hydrolysis If you need these items please order the QuickZyme Total Collagen Assay Application conditioned media cell extracts tissues tissue extracts Sensitivity 2 4 M (0 3 g/ml) Assay-time 2 hours QuickZyme Hydroxyproline Assay can be paired with QuickZyme Total Protein Assay to allow the expression of data as hydroxyproline quantity per total amount of protein